In a 24 week randomized, double-blind, placebo-controlled clinical trial, 100 adults (19-60 years) were assigned to receive either Gynostemma pentaphyllum (GP) extract or a placebo, with 94 participants completing the study. The GP extract demonstrated significant improvements in multiple hair-health parameters compared with placebo. Hair elasticity increased by 58.14 ± 40.43 gf in the GP group versus 19.92 ± 22.54 gf in the placebo group (p < 0.0001). Total hair density (hair count per cm²) increased by 14.04 ± 11.36 hairs/cm² compared to 4.85 ± 8.60 hairs/cm² in the placebo group (p < 0.0001). Hair diameter increased by 17.49 ± 6.08 μm, whereas the placebo group showed an increase of only 3.63 ± 9.16 μm (p < 0.0001). Although instrumental measurements of hair gloss did not show significant differences between groups, the extract consistently enhanced hair strength, thickness, and density. Gynostemma pentaphyllum extract is a safe and effective functional ingredient for improving hair health and supporting hair growth, offering a potential natural approach for managing hair thinning and hair loss.