A single serving of the brown seaweed extract PolySea (Ascophyllum nodosum) rapidly modulated immune and regenerative functions in healthy adults, elevating pro-inflammatory cytokines (IFN-γ, IL-6) alongside anti-inflammatory IL-1RA within 1 hour. Transient increases in monocytes and non-NK non-T cells were observed, coupled with selective mobilization of endothelial stem cells and relocation of pluripotent progenitors into tissues. These acute, coordinated immune and stem cell shifts highlight PolySea’s potential to support regenerative physiology and immune balance relevant to healthy aging.